Composite

Part:BBa_K2557011:Design

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-09)


TetO-EF1α promoter-Bxb1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 565
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1279
    Illegal BamHI site found at 499
    Illegal BamHI site found at 720
    Illegal BamHI site found at 1036
    Illegal BamHI site found at 1744
    Illegal BamHI site found at 2035
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 565
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 565
    Illegal NgoMIV site found at 1021
    Illegal AgeI site found at 505
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 741


Design Notes

SV40 NLS and flag was added by PCR.


Source

The TetO sequence was cloned from a Commercialized plasmid. The EF1αpromoter was cloned from a plasmid borrowed from other laboratories in the school. We found the coding sequence of Bxb1 on NCBI and, after humanization, sent it to Kingsray for synthesis.

References